This study aims to determine the antifungal activity of atsuete (Bixa orellana L.) hexane seed extract against Candida albicans. The extracts were dividedinto four concentrations, namely 25%, 50%. 75%. and 100%. The data was based on the zone of inhibition of each concentration. Results showed that the four concentrations exhibited a negative antifungal activity against Candida albicans. It was therefore concluded that all concentrations of teh atsuete had no effect against C. albicans.