This study aim to assess presence of biofilm populations in a drinking water dispenser through population assessment of coliform fungi, algae and protozoa. Employing the descriptive method of research, a selected drinking water dispenser from a canteen of a known state University in Iloilo City was assessed. Eosin methylene blue (EMB) and potatoe dextrose agar or (PDA) were utilized to culture population of regular non-drug-resistant coliform and fungal species whereas ciprofloxacin and fluconazole-treated culture media were used to assess populations of potential drug resistant coliform and fungi. Serial dilution and spread plate method were used to assess biofilm populations. Collected water samples from the water dispenser were subjected to microscopic examination for confirmation of the presence of algae and protozoa populations. Regular coliform populations and extant regualr and drug resistant fungal populations were found to be established in the drinking water dispenser. Moreover, the water samples were negative for algae and protozoa.