The study aimed to assess presumptive vaginal lactobacillus populations from 48 randomly selected female commercial sex workers from Iloilo City social hygience clinic. Samples were collected by a medical officer in attendance. These samples were inoculated in MRS brought on 3M petrifilm aerobic count plates and incubated for 48 hrs. Results show negative correlation between lactobacillus population with current diagnosis age and history of vaginal infections, This indicates that lactobacilli are more abundant in younger women with negative current dignosis or with no history of vaginal infections. On teh contrary, women of more advanced age, women with current vaginal infection or with history of vaginal infection were found to have lesser lactobacillus populations. The results of the study imply that lactobacillus play an importnat role in maintaining a healthy vaginal flora and against vaginal infections.